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DOI :
10.3791/54043-v
June 18th, 2016
Chapters
0:05
Title
1:44
Preparation of the Soluble Protein Fraction Containing HP-NAP
3:52
Purification of Recombinant HP-NAP from E. coli by Negative Batch Mode Chromatography with Diethylaminoethyl (DEAE) Ion-exchange Resin
6:25
Results: Negative Purification of HP-NAP by DEAE Resins in Batch Mode
8:30
Conclusion
用于重组幽门螺杆菌一步法负纯化的高产量方法,通过使用被描述在批处理模式二乙氨基树脂幽门在大肠杆菌中过表达嗜中性粒细胞激活蛋白(HP-NAP)。通过该方法纯化的HP-NAP是疫苗,药物或诊断为H的发展有利幽门螺杆菌机相关的疾病。
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