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DOI :
10.3791/54043-v
June 18th, 2016
Chapters
0:05
Title
1:44
Preparation of the Soluble Protein Fraction Containing HP-NAP
3:52
Purification of Recombinant HP-NAP from E. coli by Negative Batch Mode Chromatography with Diethylaminoethyl (DEAE) Ion-exchange Resin
6:25
Results: Negative Purification of HP-NAP by DEAE Resins in Batch Mode
8:30
Conclusion
バッチモードでジエチルアミノ樹脂を使用して、 大腸菌中で過剰発現組換えヘリコバクター・ピロリ好中球活性化タンパク質(HP-NAP)のワンステップ負精製が記載されているため、高い歩留まり方法。この方法によって精製されたHP-NAPはH.のためのワクチン、薬、または診断薬の開発のために有益ですピロリ菌は、病気を-関連しました。
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