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条纹分析来研究蛋白质底物使用游离海马神经元的吸引力或令人反感的活动

DOI :

10.3791/54096-v

June 19th, 2016

June 19th, 2016

10,264 Views

1Anatomy and Neuroscience, Hamamatsu University School of Medicine, 2Cell and Neurobiology, Zoological Institute, Karlsruhe Institute of Technology (KIT)

Axon guidance molecules regulate neuronal migration and targeted growth-cone navigation. We present a powerful method, the stripe assay, to assess the ability of guidance molecules to attract or repulse neurons. In this protocol, we demonstrate the stripe assay by showing FLRT2's ability to repel cultured hippocampal neurons.

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112 FLRT

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