Stripe Assay per studiare l'attività attrattiva o repulsiva di un substrato proteico Utilizzando dissociata neuroni dell'ippocampo

Axon guidance molecules regulate neuronal migration and targeted growth-cone navigation. We present a powerful method, the stripe assay, to assess the ability of guidance molecules to attract or repulse neurons. In this protocol, we demonstrate the stripe assay by showing FLRT2's ability to repel cultured hippocampal neurons.