Stripe Ensaio para estudar a atividade atração ou repulsão de um substrato de proteína utilizando dissociado neurônios do hipocampo

Axon guidance molecules regulate neuronal migration and targeted growth-cone navigation. We present a powerful method, the stripe assay, to assess the ability of guidance molecules to attract or repulse neurons. In this protocol, we demonstrate the stripe assay by showing FLRT2's ability to repel cultured hippocampal neurons.