Raya de ensayo para estudiar la actividad de atracción o repulsión de un sustrato de proteína usando las neuronas del hipocampo disociadas

Axon guidance molecules regulate neuronal migration and targeted growth-cone navigation. We present a powerful method, the stripe assay, to assess the ability of guidance molecules to attract or repulse neurons. In this protocol, we demonstrate the stripe assay by showing FLRT2's ability to repel cultured hippocampal neurons.