Observation of Lampbrush Chromosomes and Centrifugation of Nuclear Contents
6:33
Fixing the Nuclear Contents
7:30
Results: Visualization of Axolotl and Frog LBCs
9:14
Conclusion
Transcript
The overall goal of this procedure is to isolate the giant nucleus, or germinal vesicle, from an amphibian oocyte, and to spread the lampbrush chromosomes, and other nuclear organelles, on a microscope slide for immunostaining, in citu hybridizati
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Vi præsenterer enkle teknikker til isolering gigantiske transkriptionelt aktive børstekromosomer kromosomer fra levende oocytter af frøer og salamandre. Vi beskriver, hvordan overholdelse af disse kromosomer "live" af fasekontrast eller differentiel interferens kontrast, og hvordan man kan løse dem for fluorescerende in situ hybridisering eller immunfluorescensfarvning.