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JoVE Journal

Immunology and Infection

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Isolation and Flow Cytometric Characterization of Murine Small Intestinal Lymphocytes

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08:14 min

May 8th, 2016

May 8th, 2016

26,991 Views

0:05

Title

0:39

Preparation of a Single Cell Suspension from the Small Intestinal Intraepithelial Layer and Lamina Propria

5:22

Preparation of a Single Cell Suspension from Peyer's Patches

6:20

Results: Intestinal Lymphocytes Have FSC and SSC Characteristics Similar to Splenocytes

7:10

Conclusion

Transcript

The overall goal of this procedure is to isolate single cell populations from different small intestinal compartments that can subsequently be used for flow cytometric analysis or an alternate means of characterization. This method can help answer

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There is growing interest in the quantitative characterization of intestinal lymphocytes owing to increasing recognition that these cells play a critical role in a variety of intestinal and systemic diseases. In this protocol, we describe how to isolate single cell populations from different small-intestinal compartments for subsequent flow cytometric characterization.

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