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HPLC-based Assay to Monitor Extracellular Nucleotide/Nucleoside Metabolism in Human Chronic Lymphocytic Leukemia Cells

DOI :

10.3791/54124-v

July 20th, 2016

July 20th, 2016

10,767 Views

1Department of Medical Sciences, Human Genetics Foundation (HuGeF), University of Turin

The protocol described here represents an easy and reproducible method that employs reverse phase high-performance liquid chromatography (RP-HPLC) to measure purine metabolism on chronic lymphocytic leukemia (CLL) cells cultured under different conditions.

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