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DOI :
10.3791/54207-v
July 22nd, 2016
Chapters
0:05
Title
0:35
Preparation of Biotin-labeled RNA
2:18
Preparation of RNA-conjugated Beads
4:16
Preparation of Cellular Lysate from Primary Adipocytes
7:00
Binding and Elution of RBP
8:45
Results: RNA Pull-down Assay
10:05
Conclusion
的RNA下拉协议这里优化用于检测RNA结合蛋白(限制性商业惯例)和非编码以及编码RNA之间的相互作用。从雄激素受体(AR)的RNA片段作为例子来演示如何从初级的棕色脂肪细胞的lystate检索其RBP。
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