Reverse Transfection of siRNA and Seeding of HeLa 229 Cells
4:25
Infection of Reverse Transfected Cells
5:39
Addition of BlaM Substrate to Determine Level of Translocation
8:23
Results: FRET Measurements of Coxiella burnetti Effector Translocation Efficiency in siRNA Silenced HeLa Cells
9:37
Conclusion
Transcript
The overall goal of this experimental procedure is to examine the impact of specific host processes on the ability of bacterial pathogens to translocate effector proteins. This method can help answer key questions in the field of cellular microbio
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Investigar as interações entre patógenos bacterianos e seus hospedeiros é uma importante área de pesquisa biológica. Aqui, descrevemos as técnicas necessárias para medir efetoras translocação por Coxiella burnetii durante o silenciamento do gene siRNA usando substrato BlaM.