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Observation and Quantification of Telomere and Repetitive Sequences Using Fluorescence In Situ Hybridization (FISH) with PNA Probes in Caenorhabditis elegans

10.2K Views

10:01 min

August 4th, 2016

August 4th, 2016

10,157 Views

0:05

Title

0:37

Fixation of C. elegans

3:00

Permeabilization

4:59

Hybridization

6:42

Washing and Immunofluorescent Staining

8:41

Results: Telomere and TALT1 FISH in the Embryos

9:33

Conclusion

Transcript

The overall goal of this fluorescence in situ hybridization technique is to concisely analyze telomere number and length in C.Elegans. This method can help answer key questions about tumor regenesis, alternative lengthening of telomeres, and cellu

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We report a concise procedure of fluorescence in situ hybridization (FISH) in the gonad and embryos of Caenorhabditis elegans for observing and quantifying repetitive sequences. We successfully observed and quantified two different repetitive sequences, telomere repeats and template of alternative lengthening of telomeres (TALT).

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