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Preparation of Single-cohort Colonies and Hormone Treatment of Worker Honeybees to Analyze Physiology Associated with Role and/or Endocrine System

DOI :

10.3791/54240-v

September 6th, 2016

September 6th, 2016

15,591 Views

1Department of Biological Sciences, Graduate School of Sciences, The University of Tokyo, 2Faculty of Pharmaceutical Sciences, Doshisha Women's College

Here we describe our detailed protocol for the preparation of single-cohort honeybee colonies – a useful tool for analyzing the role-associated worker physiology. We also describe detailed protocols for treating workers with juvenile hormone and ecdysone to evaluate the involvement of these hormones in the regulation of worker behavior and/or physiology.

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