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Preparation of Formalin-fixed Paraffin-embedded Tissue Cores for both RNA and DNA Extraction

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08:30 min

August 21st, 2016

August 21st, 2016

50,362 Views

0:05

Title

0:57

Tissue Coring

2:40

Deparaffinization of the FFPE Tissue Cores

3:45

Homogenization of the Deparaffinized Cores

5:07

Digestion with Proteinase K and Nucleotide Separation

6:12

Results: Assessment of RNA and DNA Recovery and Integrity and Representative Performance in Downstream Analysis

7:50

Conclusion

Transcript

This modified extraction protocol improves RNA and DNA yields from more precisely targeted regions of interest in histopathologic tissue blocks.

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