Name: competitive transplants to evaluate hematopoietic stem cell fitness
Uploaded: 2016-08-31T13:00:00
Description: Watch this Scientific Journal Video about Competitive Transplants to Evaluate Hematopoietic Stem Cell Fitness at JoVE.com

हम यह आंकड़ा डिजाइन और प्रक्रियाओं के प्रदर्शन के साथ सहायता के लिए Roxann Hétu-कुंज के आभारी हैं। प्रयोगशाला में अनुसंधान कोल फाउंडेशन से एक संक्रमण पुरस्कार द्वारा समर्थित किया गया था, डिस्कवरी कोई अनुदान। प्राकृतिक विज्ञान और कनाडा () NSERC के इंजीनियरिंग रिसर्च काउंसिल और अभिनव के लिए कनाडा फाउंडेशन (CFI लीडर्स फंड अनुदान नहीं। 31377) से 419226-2012। Santé (FRQS) - KMH के लिए Fonds डे Recherche डु क्यूबेक एक Chercheur-BOURSIER जूनियर है।

सभी प्रक्रियाओं इस प्रोटोकॉल में वर्णित संस्थागत पशु आचार समिति द्वारा अनुमोदित किया गया है और पशु की देखभाल के दिशा-निर्देशों पर कनाडा परिषद का पालन करें। नोट: बाँझ / विशिष्ट रोगज़नक़ मुक?...

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L., Bhattacharya, D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Efficient+transplantation+via+antibody-based+clearance+of+hematopoietic+stem+cell+niches.">Efficient transplantation via antibody-based clearance of hematopoietic stem cell niches.</a> Science. 318 (5854), 1296-1299 (2007).</li><li>Zhang, C. C., Lodish, H. 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M., Chagraoui, J., Vainio, S., Sauvageau, G., Perreault, C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+signaling+protein+Wnt4+enhances+thymopoiesis+and+expands+multipotent+hematopoietic+progenitors+through+beta-catenin-independent+signaling.">The signaling protein Wnt4 enhances thymopoiesis and expands multipotent hematopoietic progenitors through beta-catenin-independent signaling.</a> Immunity. 29 (1), 57-67 (2008).</li><li>Cui, Y. Z., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Optimal+protocol+for+total+body+irradiation+for+allogeneic+bone+marrow+transplantation+in+mice.">Optimal protocol for total body irradiation for allogeneic bone marrow transplantation in mice.</a> Bone Marrow Transplant. 30 (12), 843-849 (2002).</li><li>Benz, C., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Hematopoietic+Stem+Cell+Subtypes+Expand+Differentially+during+Development+and+Display+Distinct+Lymphopoietic+Programs.">Hematopoietic Stem Cell Subtypes Expand Differentially during Development and Display Distinct Lymphopoietic Programs.</a> Cell Stem Cell. 10 (3), 273-283 (2012).</li><li>Eppert, K., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Stem+cell+gene+expression+programs+influence+clinical+outcome+in+human+leukemia.">Stem cell gene expression programs influence clinical outcome in human leukemia.</a> Nat Med. 17 (9), 1086-1093 (2011).</li><li>McIntosh, B. E., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Nonirradiated+NOD,B6.SCID+Il2rgamma-/-+Kit(W41/W41)+(NBSGW)+mice+support+multilineage+engraftment+of+human+hematopoietic+cells.">Nonirradiated NOD,B6.SCID Il2rgamma-/- Kit(W41/W41) (NBSGW) mice support multilineage engraftment of human hematopoietic cells.</a> Stem Cell Reports. 4 (2), 171-180 (2015).</li></ol>

यहाँ वर्णित प्रोटोकॉल जाना जाता प्रतियोगी एचएससी के खिलाफ दाता (परीक्षण) एचएससी के रिश्तेदार फिटनेस का मूल्यांकन करने के लिए बनाया गया है। प्रतियोगिता की स्थिति रिश्तेदार परख की संवेदनशीलता (अधिक स?...

Hematopoiesis एक पुनर्योजी प्रक्रिया है कि रक्त कोशिकाओं है कि चोट, विकिरण और कोशिका मृत्यु के माध्यम से खो दिया है की भरपाई सुनिश्चित करता है। इस प्रक्रिया hematopoietic स्टेम कोशिकाओं (एचएससी) कि मोटे तौर पर वयस्क अस्थि मज्जा में रहते द्वारा सुनिश्चित किया जाता है। इसके अलावा, hematopoietic स्टेम कोशिकाओं autoimmune विकार, रक्त कैंसर और immunodeficiencies 1 में चिकित्सीय उद्देश्यों के लिए इस्तेमाल किया जा सकता है। वहाँ इस तरह बेहतर तंत्र है कि एचएससी समारोह को विनियमित, उनके proliferative विस्तार और उनकी पहुंच और प्रत्यारोपण के बाद प्राप्तकर्ता अस्थि मज्जा टीका लगाना करने की क्षमता सहित समझने की जरूरत है। हालांकि हाल के अध्ययनों स्लैम परिवार के सदस्यों CD150 और CD48 सहित कई कोशिका की सतह मार्करों, भावी लगभग 50% शुद्धता 2-4 को वयस्क एचएससी और भ्रूण एचएससी संतुष्ट करने के लिए सूचित किया है, कार्यात्मक एचएससी के लिए स्वर्ण मानक मापने के लिए एक विवो repopulating परख बनी हुई है उनकी फिर से स्थापित करने के लिए रक्त ग क्षमता का निर्धारणएक विकिरणित मेजबान 5 में पक्ष उत्पादन। इन विवो प्रतिरूप repopulating परख शुरू तक और McCulloch 6 से विकसित किया गया था और तब से परिष्कृत और विस्तारित किया गया है। मूल रूप में परिभाषित किया गया, एचएससी आत्म नवीकरण और भेदभाव के माध्यम से आजीवन रक्त कोशिका के उत्पादन किया जाता है। एक विकिरणित प्राप्तकर्ता में एचएससी के हस्तांतरण इस प्रकार हमें आकलन करने के लिए अनुमति देता है: उनके अलग अलग रक्त कोशिका प्रजातियों के विश्लेषण के माध्यम से अंतर करने के लिए (टी lymphocytes, बी लिम्फोसाइट, granulocytes, monocytes) और धारावाहिक प्रत्यारोपण के माध्यम से आत्म नवीकरण के लिए अपनी क्षमता की क्षमता। परख आमतौर पर कार्यक्षमता और / या एचएससी की दो आबादी की मात्रा की तुलना शामिल होगा, जैसे, अलग जीनोटाइप या कोशिकाओं के दो चूहों कि इलाज किया गया है या विभिन्न कारकों है कि रखरखाव या एचएससी के विस्तार को प्रभावित कर सकता है के साथ इलाज से आ रही कोशिकाओं संस्कृति में। डोनर काइमेरावाद, या स्थानांतरित कर दाता एचएससी टी का योगदानओ रक्त कोशिका के उत्पादन तो कोशिका की सतह मार्करों या अन्य तरीकों कि प्राप्तकर्ता, या मेजबान से दाता कोशिकाओं का उपयोग कर अलग होगा परिधीय रक्त में प्रवाह cytometry विश्लेषण और अस्थि मज्जा के द्वारा निर्धारित किया जा सकता है। सबसे व्यापक रूप से इस्तेमाल किया मार्करों निश्चित रूप से जीन Ptprc या CD45 ल्युकोसैट प्रतिजन 7 कि हम नीचे दिए गए उदाहरणों के लिए चुना है के लिए दो alleles हैं। प्रतिरूप repopulation परख सकते हैं या तो प्रतियोगी या गैर-प्रतिस्पर्धी हो सकते हैं। एक गैर-प्रतिस्पर्धी माहौल में, नियंत्रण और परीक्षण एचएससी अलग प्राप्तकर्ता चूहों में स्थानांतरित कर रहे हैं और प्रत्येक कोशिका प्रकार के लिए परिणाम दूसरे से स्वतंत्र होगी। एक प्रतिस्पर्धी माहौल में, दोनों परीक्षण और नियंत्रण एचएससी के समारोह प्रतियोगी एचएससी की आबादी के खिलाफ मापा जाता है। यहां वर्णित प्रोटोकॉल प्रतिस्पर्धी सेटिंग का उपयोग करता है, लेकिन यह भी गैर-प्रतिस्पर्धी स्थितियों के लिए अनुकूलित किया जा सकता है। दोनों दृष्टिकोण के अपने फायदे और सीमाएं हैं, और हम में विस्तार से उनकी तुलना होगाचर्चा। हम यह भी प्रत्यारोपित एचएससी की संख्या में इक्विटी सुनिश्चित करने के लिए अलग अलग दृष्टिकोण का वर्णन है, कैसे कमजोर पड़ने परख (झील प्राधिकरण) को सीमित करके एचएससी की मात्रा का ठहराव के लिए परख के लिए अनुकूल है, और परिणाम की व्याख्या के लिए दोनों सफल और असफल प्रत्यारोपण का उदाहरण देते समझाओ।

<table border="0" cellpadding="0" cellspacing="0" width="703">
	<tbody>
		<tr height="44">
			<td height="44" style="height:44px;width:216px;">Microtainer tubes with K2EDTA</td>
			<td style="width:89px;">BD Biosciences</td>
			<td style="width:112px;"></td>
			<td style="width:119px;">365974</td>
			<td style="width:167px;"></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">20G needle</td>
			<td style="width:89px;">BD Syringe</td>
			<td style="width:112px;"></td>
			<td style="width:119px;"></td>
			<td>For blood sampling from the mandibular vein</td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;width:216px;">LabQuake Shaker rotisserie</td>
			<td style="width:89px;">Thermo&#160; Scientific</td>
			<td style="width:112px;"></td>
			<td style="width:119px;">C415110</td>
			<td>Any other rotating mixer will work as well to prevent coagulation of blood samples</td>
		</tr>
		<tr height="63">
			<td height="63" style="height:63px;width:216px;">Purified anti-mouse CD16/CD32 (clone 2.4G2, Fc Block)</td>
			<td style="width:89px;">BD Biosciences</td>
			<td style="width:112px;">2.50</td>
			<td style="width:119px;">553142</td>
			<td>Alternatively use clone 93 from eBioscience (cat # 14-0161) or Biolegend (cat# 101310)&#160;</td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;width:216px;">Pe-Cy7-conjugated anti-mouse CD3e (clone 145-2C11)</td>
			<td style="width:89px;">eBioscience</td>
			<td style="width:112px;">0.25</td>
			<td style="width:119px;">25-0031</td>
			<td>For most flow cytometry antibodies, the clone is important but the colours and companies can vary depending on the available equipment</td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;width:216px;">PE-conjugated anti-mouse CD19 (clone 1D3)</td>
			<td style="width:89px;">eBioscience</td>
			<td style="width:112px;">0.25</td>
			<td style="width:119px;">12-0193</td>
			<td></td>
		</tr>
		<tr height="63">
			<td height="63" style="height:63px;width:216px;">APC-eFluor780 (APC-Cy7 equivalent)-conjugated anti-mouse GR1 (clone RB6-8C5)</td>
			<td style="width:89px;">eBioscience</td>
			<td style="width:112px;">0.25</td>
			<td style="width:119px;">47-5931</td>
			<td></td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;width:216px;">FITC-conjugate anti-mouse CD45.1 (clone A20)</td>
			<td style="width:89px;">eBioscience</td>
			<td style="width:112px;">2.50</td>
			<td style="width:119px;">11-0453</td>
			<td></td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;width:216px;">eFluor450-conjugated anti-mouse CD45.2 (clone 104)</td>
			<td style="width:89px;">eBioscience</td>
			<td style="width:112px;">1.00</td>
			<td style="width:119px;">48-0454</td>
			<td></td>
		</tr>
		<tr height="63">
			<td height="63" style="height:63px;width:216px;">Biotinylated anti-human/mouse CD45R (B220) (clone RA3-6B2)</td>
			<td style="width:89px;">eBioscience</td>
			<td style="width:112px;">1.25</td>
			<td style="width:119px;">13-0452</td>
			<td></td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;width:216px;">Biotinylated anti-mouse CD3e (clone 145-2C11)</td>
			<td style="width:89px;">eBioscience</td>
			<td style="width:112px;">1.25</td>
			<td style="width:119px;">13-0031</td>
			<td></td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;width:216px;">Biotinylated anti-mouse CD11b (clone M1/70)</td>
			<td style="width:89px;">eBioscience</td>
			<td style="width:112px;">1.25</td>
			<td style="width:119px;">13-0112</td>
			<td></td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;width:216px;">Biotinylated anti-mouse GR1 (clone RB6-8C5)</td>
			<td style="width:89px;">eBioscience</td>
			<td style="width:112px;">1.25</td>
			<td style="width:119px;">13-5931</td>
			<td></td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;width:216px;">Biotinylated anti-mouse TER119 (clone TER119)</td>
			<td style="width:89px;">eBioscience</td>
			<td style="width:112px;">0.63</td>
			<td style="width:119px;">13-5921</td>
			<td></td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;width:216px;">V500 streptavidin</td>
			<td style="width:89px;">BD Biosciences</td>
			<td style="width:112px;">0.50</td>
			<td style="width:119px;">561419</td>
			<td></td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;width:216px;">PE-conjugated anti-mouse CD117 (clone 2B8)</td>
			<td style="width:89px;">BD Biosciences</td>
			<td style="width:112px;">0.25</td>
			<td style="width:119px;">553355</td>
			<td></td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;width:216px;">PE-Cy7-conjugated anti-mouse Ly6A/E (Sca1) (clone D7)</td>
			<td style="width:89px;">BD Biosciences</td>
			<td style="width:112px;">0.25</td>
			<td style="width:119px;">558162</td>
			<td></td>
		</tr>
		<tr height="63">
			<td height="63" style="height:63px;width:216px;">PerCP-eFluor710-conjugated anti-mouse CD135 (clone A2F10)</td>
			<td style="width:89px;">eBioscience</td>
			<td style="width:112px;">0.50</td>
			<td style="width:119px;">46-1351</td>
			<td></td>
		</tr>
		<tr height="63">
			<td height="63" style="height:63px;width:216px;">Alexa fluor 647-conjugated anti-mouse CD150 (clone TC15-12F12.2)</td>
			<td style="width:89px;">Biolegend</td>
			<td style="width:112px;">0.63</td>
			<td style="width:119px;">115918</td>
			<td>BD Biosciences and eBioscience do not carry the same clone</td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;width:216px;">1ml tuberculin syringe with 27G needle</td>
			<td style="width:89px;">BD Syringe</td>
			<td style="width:112px;"></td>
			<td style="width:119px;">309623</td>
			<td></td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;width:216px;">1ml tuberculin syringe with 25G needle</td>
			<td style="width:89px;">BD Syringe</td>
			<td style="width:112px;"></td>
			<td style="width:119px;">309626</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">70 um cell strainer</td>
			<td style="width:89px;">BD Falcon</td>
			<td style="width:112px;"></td>
			<td style="width:119px;">352350</td>
			<td></td>
		</tr>
	</tbody>
</table>

competitive transplants to evaluate hematopoietic stem cell fitness

The gold standard definition of a hematopoietic stem cell (HSC) is a cell that when transferred into an irradiated recipient will have the ability to reestablish blood cell production for the lifespan of the recipient. This protocol explains how to set up a functional assay to compare the HSC capacities of two different populations of cells, such as bone marrow from mice of two different genotypes, and how to analyze the recipient mice by flow cytometry. The protocol uses HSC equivalents rather than cell sorting for standardization and discusses the advantages and disadvantages of both approaches. We further discuss different variations to the basic protocol, including serial transplants, limiting dilution assays, homing assays and non-competitive transplants, including the advantages and preferred uses of these varied approaches. These assays are central for the study of HSC function and could be used not only for the investigation of fundamental HSC intrinsic aspects of biology but also for the development of preclinical assays for bone marrow transplant and HSC expansion in culture.

माध्यमिक प्रत्यारोपण (आगे नीचे) पर चर्चा सहित प्रतिस्पर्धी प्रत्यारोपण की स्थापना की एक सामान्य विवरण दोहरी का बहिष्कार पर चित्रा 1 में पाया जा सकता है। पूर्व प्रत्यारोपण अस्थि मज?...

Watch this Scientific Journal Video about Competitive Transplants to Evaluate Hematopoietic Stem Cell Fitness at JoVE.com

Competitive Transplants to Evaluate Hematopoietic Stem Cell Fitness

This protocol provides step-by-step guidelines for setting up competitive mouse bone marrow transplant experiments to study hematopoietic stem/progenitor cell function without prior purification of stem cells by cell sorting.

प्रतियोगी प्रत्यारोपण hematopoietic स्टेम सेल स्वास्थ्य मूल्यांकन करने के लिए

The gold standard definition of a hematopoietic stem cell (HSC) is a cell that when transferred into an irradiated recipient will have the ability to ...

The overall goal of this experiment is to determine the fitness of bone marrow hematopoietic stem cells in vivo using a competitive transplant assay. So this method can help answer key questions in the field of stem cell biology and hematopoietic cell transplantation about the impact of various manipulations on stem cell self-renewal after transplant. The main advantages of this technique are that it requires a limited number of animals and that it does not involve cell sorting.Though this method can provide insight into stem cell function, it can be also useful in other fields such as immunology, through the use of mixed bone marrow chimeras. The initial development of this technique in the 1960s paved the way for researchers in the field of stem cell biology to explore the identity and function of hematopoietic stem cells in humans and mice. Demonstrating the procedure will be Roxanne Hetu-Arbour, a Master's student in the lab, and Edward Owusu Kwarteng, a PhD student.Begin by placing the mouse inside a biological safety cabinet on a surgical platform and wet the skin and fur with 70%ethanol. Next, take hold of a hind foot and cut the skin up along the leg. Proceed to pull away the skin and cut away the excess muscle.Then, with the scissor blades against the pelvic bones as a counter-force, pull on the leg bone to dislocate the femur. Detach the tibia and the femur from the kneecap, then remove the foot, and remove the remaining bits of muscle and tendons. Then, place the bones in two to three milliliters of sterile PBS in one well of a six-well tissue culture plate.When all of the bones have been harvested, grasp the bones one at a time with a forceps, and insert a 25-gauge needle attached to a one-milliliter syringe filled with sterile PBS into one end of the bone. Depress the plunger, collecting the cells in a 15-milliliter conical tube until the center of each bone is white. Using a 22-gauge needle, dissociate the cells into a single cell suspension by repeatedly passing them through the needle tip.After all of the bone marrow has been collected, filter the cells through a 70-micron nylon strainer and count the resulting cell suspensions. Then, centrifuge the bone marrow cells, remove the supernatant, and dilute the samples to a one times ten to the eighth cells per milliliter concentration in fresh PBS, on ice. To establish donor hematopoietic stem cell equivalents, transfer 30 microliter aliquots of cells into five-milliliter round-bottomed polystyrene tubes and add an equal volume of unlabeled antibody against CD16 and CD32 to the samples for five minutes at room temperature to block any non-specific staining.Next, incubate the cells in 90 microliters of the appropriate fluorochrome conjugated primary antibody master mix on ice, protected from light. After 30 minutes, add two milliliters of staining buffer to the cells and vortex the tubes. Follow this with centrifugation.Decant the supernatants and resuspend the pellets by flicking. Then, label the cells with 10 microliters of fluorochrome conjugated streptavidin on ice for 20 minutes in the dark. At the end of the incubation, wash the cells in two more milliliters of buffer and analyze the samples by flow cytometry.Using one sample as a baseline, establish the estimated hematopoietic stem cell equivalents for each of the samples. After calculating the number of hematopoietic stem cells per sample as outlined in the text, mix the competitor and test hematopoietic stem cells at a one-to-one ratio and adjust the final volume of the cells to 200 microliters per injection in sterile PBS. For adoptive transfer of the hematopoietic stem cells, warm up an irradiated recipient mouse with a heat lamp.Then aspirate 750 microliters of cells into one one-milliliter tuberculin syringe equipped with a 27-gauge needle and remove the bubbles. Next, with the mouse in the appropriate restraining device, wipe the injection site with ethanol. Insert the needle into a lateral tail vein, bevel side up and parallel to the skin, and gently depress the plunger.When all of the cells have been injected, remove the needle and compress the injection site with sterile gauze for a few seconds to stop the bleeding. Then place the mouse in a clean cage. To analyze the peripheral blood, pierce the facial skin near the hairless spot located under the jawbone and collect one to two drops of blood in an EDTA tube.Place the tube in a rotator to prevent clotting while waiting to proceed. Next, mix one milliliter of freshly-prepared, room temperature, red blood cell lysis buffer with the blood and transfer the sample to a five-milliliter, round-bottomed polystyrene tube. After four minutes at room temperature, add four volumes of ice-cold PBS to each tube and cover the opening with a cap.Mix the tube a few times by inversion and immediately place the cells on ice. After centrifugation, decant the clear red supernatants and resuspend the pellets by flicking. Then add two milliliters of staining buffer to the cells and briefly vortex them.Follow this with a a second centrifugation. Resuspend the pellets by flicking and proceed with flow cytometry staining as detailed in the text. The competitor-derived cells should be present in all three of the myeloid, B-lymphoid, and T-lymphoid lineages.The competitor and donor cells are also found within both lineage negative and hematopoietic stem cell bone marrow cell populations. When the donor hematopoietic stem cells are functionally equivalent to the competitor cells, the proportions of the donor and the competitor-derived cells are equal. The residual host cells are typically almost exclusively T lymphocytes, as these cells are more radioresistant.When the donor cells are observed at a much lower proportion of peripheral blood leukocytes than the competitor cells, some aspects of hematopoietic stem cell function are defective, and warrant further investigation. When the donor and competitor cells are both present in low numbers and the host cells represent the majority of peripheral blood cells, the transplant is considered unsuccessful, and no conclusions can be drawn about the relative functionality of the donor versus the competitor cells. Once mastered, the bone marrow preparation and the transplantation steps can be completed in six to seven hours if they are performed properly.While attempting this procedure, it is important to remember to maintain sterile conditions during the cell preparation and the animal manipulation steps. Following this procedure, other methods, like a limiting dilution assay, can be performed to answer additional questions regarding the functional stem cell numbers. After watching this video, you should have a good understanding of how to perform competitive bone marrow transplants.Don't forget that working with irradiation can be extremely hazardous, and that the appropriate precautions should always be taken while performing this procedure.

Research

JoVE Journal

Developmental Biology

Direct Induction of Human Neural Stem Cells from Peripheral Blood Hematopoietic Progenitor Cells

परिधीय रक्त Hematopoietic पूर्वपुस्र्ष कोशिकाओं से मानव तंत्रिका स्टेम कोशिकाओं के प्रत्यक्ष प्रेरण

Human disease specific neuronal cultures are essential for generating in vitro models for human neurological diseases. However, the lack of access to ...

Automated Quantification of Hematopoietic Cell &#8211; Stromal Cell Interactions in Histological Images of Undecalcified Bone

Hematopoietic सेल की स्वचालित मात्रा - Undecalcified अस्थि के histological छवियाँ में stromal सेल सहभागिता

Confocal microscopy is the method of choice for the analysis of localization of multiple cell types within complex tissues such as the bone marrow. ...

Generation of Genetically Modified Organotypic Skin Cultures Using Devitalized Human Dermis

निष्प्राण मानव डर्मिस का प्रयोग आनुवंशिक रूप से संशोधित Organotypic त्वचा संस्कृतियों की पीढ़ी

Organotypic cultures allow the reconstitution of a 3D environment critical for cell-cell contact and cell-matrix interactions which mimics the function ...

Directed Differentiation of Primitive and Definitive Hematopoietic Progenitors from Human Pluripotent Stem Cells

मानव Pluripotent स्टेम सेल से आदिम और निश्चित टेम Progenitors के विभेद का निर्देशन

One of the major goals for regenerative medicine is the generation and maintenance of hematopoietic stem cells (HSCs) derived from human pluripotent stem ...

A Novel Mammary Fat Pad Transplantation Technique to Visualize the Vessel Generation of Vascular Endothelial Stem Cells

संवहनी एंडोथिलियल स्टेम सेल के पोत जनरेशन को विज़ुअलाइज़ करने के लिए एक उपन्यास स्तनधारी फैट पैड ट्रांसप्लांटेशन तकनीक

Endothelial cells (ECs) are the fundamental building blocks of the vascular architecture and mediate vascular growth and remodeling to ensure proper ...

Detection of Residual Donor Erythroid Progenitor Cells after Hematopoietic Stem Cell Transplantation for Patients with Hemoglobinopathies

Hemoglobinopathies के साथ रोगियों के लिए टेम स्टेम कोशिका प्रत्यारोपण के बाद अवशिष्ट दाता Erythroid जनक कोशिकाओं का पता लगाने

The presence of incomplete chimerism is noted in a large proportion of patients following bone marrow transplant for thalassemia major or sickle cell ...

Development of an In Vitro Assay to Quantitate Hematopoietic Stem and Progenitor Cells (HSPCs) in Developing Zebrafish Embryos

Development of an In Vitro Assay to Quantitate Hematopoietic Stem and Progenitor Cells HSPCs in Developing Zebrafish Embryos

एक इन विट्रो परख के विकास के लिए Quantitate टेम स्टेम और जनक कोशिकाओं (HSPCs) Zebrafish भ्रूण के विकास में

Hematopoiesis is an essential cellular process in which hematopoietic stem and progenitor cells (HSPCs) differentiate into the multitude of different cell ...

Clonal Analysis of Embryonic Hematopoietic Stem Cell Precursors Using Single Cell Index Sorting Combined with Endothelial Cell Niche Co-culture

भ्रूण टेम के क्लोनल विश्लेषण स्टेम सेल Endothelial सेल आला सह संस्कृति के साथ संयुक्त छंटाई एकल कोशिका सूचकांक का उपयोग कर पुरोगामी

The ability to study hematopoietic stem cell (HSC) genesis during embryonic development has been limited by the rarity of HSC precursors in the early ...

Use of Hematopoietic Stem Cell Transplantation to Assess the Origin of Myelodysplastic Syndrome

Myelodysplastic सिंड्रोम की उत्पत्ति का आकलन करने के लिए टेम स्टेम कोशिका प्रत्यारोपण का उपयोग

Myelodysplastic syndromes (MDS) are a diverse group of hematopoietic stem cell disorders that are defined by ineffective hematopoiesis, peripheral blood ...