Name: potentiodynamic corrosion testing
Uploaded: 2016-09-04T14:00:00
Description: Watch this Scientific Journal Video about Potentiodynamic Corrosion Testing at JoVE.com

The authors had no funding provided for this study.

1. Konstruktion af Sample Holder <ol><li> Konstruere holder prøven fra rustfrit stål afstandsstykker og en M3 rustfrit stål gevind skrue, holdes på plads med en M3 sekskantet møtrik. </li><li> Fjern hoved gevind skruen med en tang og polere snittet segment at fastholde tråden mønster. </li><li> Når alle individuelle komponenter er klar, samle elektrodeholdere. Hver holder elektrode indeholder tre afstandsstykker forbundet med hinanden ved de M3 skruer resulterer i en 11,5 cm skaft. Placer sekskantede møtrikke...

<ol>
	<li>Isaacs, H. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Aspects+of+corrosion+from+the+ECS+Publications.">Aspects of corrosion from the ECS Publications.</a> J. Electrochem. Soc. 149 (12), 85-87 (2002).</li><li>Fontana, M. G., Greene, N. D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=.">.</a> Corrosion Engineering. , (1978).</li><li>Pourbaix, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Electrochemical+corrosion+of+metallic+biomaterials.">Electrochemical corrosion of metallic biomaterials.</a> Biomaterials. 5 (3), 122-134 (1984).</li><li>Rechnitz, G. 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M., Wilkinson, J. M., Ferner, R. E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Systemic+toxicity+related+to+metal+hip+prostheses.">Systemic toxicity related to metal hip prostheses.</a> Clin Toxicol (Phila). 52 (8), 837-847 (2014).</li><li>Davda, K., Lali, F. V., Sampson, B., Skinner, J. A., Hart, A. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=An+analysis+of+metal+ion+levels+in+the+joint+fluid+of+symptomatic+patients+with+metal-on-metal+hip+replacements.">An analysis of metal ion levels in the joint fluid of symptomatic patients with metal-on-metal hip replacements.</a> J Bone Joint Surg Br. 93 (6), 738-745 (2011).</li><li>Clarke, M. T., Lee, P. T., Arora, A., Villar, R. N. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Levels+of+metal+ions+after+small+and+large+diameter+metal-on-metal+hip+arthroplasty.">Levels of metal ions after small and large diameter metal-on-metal hip arthroplasty.</a> J Bone Joint Surg Br. 85 (6), 913-917 (2003).</li><li>Brown, S. A., Hughes, P. J., Merritt, K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=In+vitro+studies+of+fretting+corrosion+of+orthopaedic+materials.">In vitro studies of fretting corrosion of orthopaedic materials.</a> J Orthop Res. 6 (4), 572-579 (1988).</li><li>Bryant, M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Characterisation+of+the+surface+topography,+tomography+and+chemistry+of+fretting+corrosion+product+found+on+retrieved+polished+femoral+stems.">Characterisation of the surface topography, tomography and chemistry of fretting corrosion product found on retrieved polished femoral stems.</a> J Mech Behav Biomed Mater. 32, 321-334 (2014).</li><li>Jantzen, C., J&#248;rgensen, H. L., Duus, B. R., Sporring, S. L., Lauritzen, J. B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Chromium+and+cobalt+ion+concentrations+in+blood+and+serum+following+various+types+of+metal-on-metal+hip+arthroplasties.+A+literature+review.">Chromium and cobalt ion concentrations in blood and serum following various types of metal-on-metal hip arthroplasties. A literature review.</a> Acta Orthopaedica. 84 (3), 229-236 (2013).</li><li>Campbell, P., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Histological+Features+of+Pseudotumor-like+Tissues+From+Metal-on-Metal+Hips.">Histological Features of Pseudotumor-like Tissues From Metal-on-Metal Hips.</a> Clin. Orthop. Relat. Res. 468 (9), 2321-2327 (2010).</li><li>Cook, S. D., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+in+vivo+performance+of+250+internal+fixation+devices:+a+follow-up+study.">The in vivo performance of 250 internal fixation devices: a follow-up study.</a> Biomaterials. 8 (3), 177-184 (1987).</li><li>Cooper, H. J., Urban, R. M., Wixson, R. L., Meneghini, R. M., Jacobs, J. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Adverse+local+tissue+reaction+arising+from+corrosion+at+the+femoral+neck-body+junction+in+a+dual-taper+stem+with+a+cobalt-chromium+modular+neck.">Adverse local tissue reaction arising from corrosion at the femoral neck-body junction in a dual-taper stem with a cobalt-chromium modular neck.</a> J Bone Joint Surg Am. 95 (10), 865-872 (2013).</li><li>Langton, D. J., Sprowson, A. P., Joyce, T. J., Reed, M., Carluke, I., Partington, P., Nargol, A. V. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Blood+metal+ion+concentrations+after+hip+resurfacing+arthroplasty.">Blood metal ion concentrations after hip resurfacing arthroplasty.</a> J Bone Joint Surg Br. 91 (10), 1287-1295 (2009).</li><li>Langton, D. J., Jameson, S. S., Joyce, T. J., Webb, J., Nargol, A. V. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+effect+of+component+size+and+orientation+on+the+concentrations+of+metal+ions+after+resurfacing+arthroplasty+of+the+hip.">The effect of component size and orientation on the concentrations of metal ions after resurfacing arthroplasty of the hip.</a> J Bone Joint Surg Br. 90 (9), 1143-1151 (2008).</li><li>Daniel, J., Ziaee, H., Pradhan, C., McMinn, D. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Six-year+results+of+a+prospective+study+of+metal+ion+levels+in+young+patients+with+metal-on-metal+hip+resurfacings.">Six-year results of a prospective study of metal ion levels in young patients with metal-on-metal hip resurfacings.</a> J Bone Joint Surg Br. 91 (2), 176-179 (2009).</li><li>De Haan, R., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Correlation+between+inclination+of+the+acetabular+component+and+metal+ion+levels+in+metal-on-metal+hip+resurfacing+replacement.">Correlation between inclination of the acetabular component and metal ion levels in metal-on-metal hip resurfacing replacement.</a> J Bone Joint Surg Br. 90 (10), 1291-1297 (2008).</li><li>Dijkman, M. A., de Vries, I., Mulder-Spijkerboer, H., Meulenbelt, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Cobalt+poisoning+due+to+metal-on-metal+hip+implants.">Cobalt poisoning due to metal-on-metal hip implants.</a> Ned Tijdschr Geneeskd. 156 (42), A4983 (2012).</li><li>Fisher, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Bioengineering+reasons+for+the+failure+of+metal-on-metal+hip+prostheses:+an+engineer's+perspective.">Bioengineering reasons for the failure of metal-on-metal hip prostheses: an engineer's perspective.</a> J Bone Joint Surg Br. 93 (8), 1001-1004 (2011).</li><li>Goldberg, J. R., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+Multicenter+Retrieval+Study+of+the+Taper+Interfaces+of+Modular+Hip+Prostheses.">A Multicenter Retrieval Study of the Taper Interfaces of Modular Hip Prostheses.</a> Clin. Orthop. Relat. Res. (401), 149-161 (2002).</li><li>Ingham, E., Fisher, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Biological+reactions+to+wear+debris+in+total+joint+replacement.">Biological reactions to wear debris in total joint replacement.</a> Proc Inst Mech Eng H. 214 (1), 21-37 (2000).</li><li>Gilbert, J. L., Buckley, C. A., Jacobs, J. J., Res, J. .. B. i. o. m. e. d. .. M. a. t. e. r. .. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=In+vivo+corrosion+of+modular+hip+prosthesis+components+in+mixed+and+similar+metal+combinations.+The+effect+of+crevice,+stress,+motion+and+allot+coupling.">In vivo corrosion of modular hip prosthesis components in mixed and similar metal combinations. The effect of crevice, stress, motion and allot coupling.</a> J. Biomed. Mater. Res. 76 (1), 1533-1544 (1993).</li><li>Browne, J. A., Bechtold, C. D., Berry, D. J., Hanssen, A. D., Lewallen, D. G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Failed+metal-on-metal+hip+arthroplasties:+a+spectrum+of+clinical+presentations+and+operative+findings.">Failed metal-on-metal hip arthroplasties: a spectrum of clinical presentations and operative findings.</a> Clin. Orthop. Relat. Res. 468 (9), 2313-2320 (2010).</li><li>Jantzen, C., Jorgensen, H. L., Duus, B. R., Sporring, S. L., Lauritzen, J. B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Chromium+and+cobalt+ion+concentrations+in+blood+and+serum+following+various+types+of+metal-on-metal+hip+arthroplasties:+a+literature+overview.">Chromium and cobalt ion concentrations in blood and serum following various types of metal-on-metal hip arthroplasties: a literature overview.</a> Acta Orthop. 84 (3), 229-236 (2013).</li><li>Frangini, S., De Cristofaro, N. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Analysis+of+galvanostatic+polarisation+method+for+determining+reliable+pitting+potentials+on+stainless+steels+in+crevice-free+conditions.">Analysis of galvanostatic polarisation method for determining reliable pitting potentials on stainless steels in crevice-free conditions.</a> Corros Sci. 45 (12), 2769-2786 (2002).</li></ol>

Polarisering scanninger fremstillet af rustfrit stål prøver viste ren kontinuerlige plots korrelerer med scanninger set i litteraturen indikerer en velfungerende korrosion system, som er både pålidelig og reproducerbar 29. Dårlig reproducerbarhed potentiodynamic pitting potentialer identificeres med en spredning af et par hundrede millivolt, med grubetæring potentiel væsen præget af en stokastisk proces 29. Dette er almindeligvis på grund af de variable af temperatur, halogenid indhold og ...

Elektrokemiske teknikker giver en hurtig og forholdsvis billig metode til at opnå de elektrokemiske egenskaber ved et materiale. Disse teknikker er baseret overvejende på evnen til at påvise korrosion af et metal ved at observere reaktion af ladningen-overførsel proces til en kontrolleret elektrokemisk forstyrrelse 1-5. Korrosion af metal implantater i en krop miljø er afgørende på grund af de negative følger for biokompatibilitet og materiale integritet 6. Den vigtigste faktor, der bidrager til korrosion af implantater i kroppen er opløsningen af overfladen oxid fører til en øget frigivelse af metalioner 7-11. Dette resulterer i uønskede biologiske reaktioner, som kan findes lokalt, men med potentielt systemiske virkninger, der fører til tidlig svigt af implantatet 10,12-28. De korrosion karakteristika et prøveemne forudsiges fra polarisering scanning produceredeaf en potentiostat. En polarisering scanning giver mulighed for ekstrapolering af de kinetiske og ætsning parametre for en metal substrat. Under en scanning, kan oxidationen eller reduktionen af ​​en elektro-aktive arter begrænses ved ladningsoverførsel og bevægelsen af ​​reaktanter eller produkter. Disse faktorer er alle indkapslet af polarisering scanning; derfor vigtigheden af ​​at have et system, der producerer en pålidelig og gentagelig polarisering scanning på tværs af flere cykler er af stor betydning. Hovedfokus i dette manuskript er at give en protokol identificerer rationale og tages skridt til at opnå et velfungerende potentiodynamic korrosion system.

<table border="0" cellpadding="0" cellspacing="0" width="573">
	<tbody>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Potentiostat</td>
			<td style="width:71px;">Metrohm</td>
			<td style="width:119px;">PGSTAT101</td>
			<td style="width:167px;"></td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;width:216px;">Ag/AgCl reference electrode, shielded</td>
			<td style="width:71px;">Metrohm</td>
			<td style="width:119px;">6.0729.100</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Electrode shaft</td>
			<td style="width:71px;">Metrohm</td>
			<td style="width:119px;">6.1241.060</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Polisher Forcipol 1v</td>
			<td style="width:71px;">Metkon</td>
			<td align="right" style="width:119px;">3602</td>
			<td></td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;width:216px;">Clindrical flask 700mL</td>
			<td style="width:71px;">SciLabware</td>
			<td style="width:119px;">FR700F</td>
			<td></td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;width:216px;">Reaction lid</td>
			<td style="width:71px;">SciLabware</td>
			<td style="width:119px;">MAF2/41</td>
			<td></td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;width:216px;">Dichloromethane</td>
			<td style="width:71px;">Sigma-Aldrich</td>
			<td style="width:119px;">MKBR7629V</td>
			<td>use under a fumehood. Wear protective clothing</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Thermo / HAAKE D Series Immersion Circulators</td>
			<td style="width:71px;">Haake</td>
			<td style="width:119px;"></td>
			<td></td>
		</tr>
	</tbody>
</table>

potentiodynamic corrosion testing

Different metallic materials have different polarization characteristics as dictated by the open circuit potential, breakdown potential, and passivation potential of the material. The detection of these electrochemical parameters identifies the corrosion factors of a material. A reliable and well-functioning corrosion system is required to achieve this.
Corrosion of the samples was achieved via a potentiodynamic polarization technique employing a three-electrode configuration, consisting of reference, counter, and working electrodes. Prior to commencement a baseline potential is obtained. Following the stabilization of the corrosion potential (Ecorr), the applied potential is ramped at a slow rate in the positive direction relative to the reference electrode. The working electrode was a stainless steel screw. The reference electrode was a standard Ag/AgCl. The counter electrode used was a platinum mesh. Having a reliable and well-functioning in vitro corrosion system to test biomaterials provides an in-expensive technique that allows for the systematic characterization of the material by determining the breakdown potential, to further understand the material's response to corrosion. The goal of the protocol is to set up and run an in vitro potentiodynamic corrosion system to analyze pitting corrosion for small metallic medical devices.

Ved afslutningen af den procedure, et in vitro korrosion systemet er indstillet til at foretage korrosion undersøgelser. Særlige procedurer såsom rengøring af korrosion skibet og Faradays bur blev indført i protokollen for at forbedre støj ydeevne. Det grundlæggende koncept af en god polarisering scanning er at identificere de elektro-fysiske tilstande af materialet giver værdifuld information for at forstå den korrosion modtagelighed af et metallisk materiale. Procedur...

Watch this Scientific Journal Video about Potentiodynamic Corrosion Testing at JoVE.com

Potentiodynamic Corrosion Testing

Here, we present a protocol to set up and run an in vitro potentiodynamic corrosion system to analyze pitting corrosion for small metallic medical devices.

Potentiodynamic Korrosionsprøvning

Different metallic materials have different polarization characteristics as dictated by the open circuit potential, breakdown potential, and passivation ...

The overall goal of this procedure is to provide a reliable setup used to carry out potentiodynamic corrosion on small metallic samples. This method can answer key questions in the biomedical field, such as being able to deduce the corrosion susceptibility of implant or materials. The main advantage of this technique is that it is a relatively inexpensive method of corroding small metallic samples.Construct the sample holder from stainless steel spacers and an M3 stainless-steel threaded screw held in place with an M3 hexagonal nut. Remove the head of the threaded screw using pliers, and polish the cut segment to maintain the thread pattern. When all individual components are ready, assemble the electrode holders.Each electrode holder contains three spacers joined together by the M3 screws, resulting in an 11.5 centimeter handle. Place the hexagonal nuts at the junction of the screw and spacers to lock the connection. Once the electrode holders are assembled, apply multiple coats of stop-off lacquer to prevent the stainless steel rods from corroding while immersed in the corrosion chamber.To do this, quickly coat half of each electrode holder and allow it to dry before coating the other half. This will help obtain a complete well-sealed coat without damaging the areas to be coated. Place the electrodes in an elevated position in the fume hood to dry.Ensure the freshly coated regions do not touch other surfaces, as this will ruin the applied coat. Clean the corrosion vessel before every corrosion run. Scrub the vessel with household detergent and rinse thoroughly with tap water.Repeat this step three times. Then, rinse the corrosion vessel three times with deionized water to remove potential contaminants found in tap water. Once rinsing with deionized water is completed, pour 300 milliliters of 95%ethanol into the corrosion vessel and swirl around to contact all internal surfaces.Pour out the ethanol and repeat this step three times. Leave the corrosion vessel under a fume hood for 30 minutes to allow all of the ethanol to completely evaporate before rinsing with the electrolyte to be used for the corrosion run, as described in the text protocol. The working electrode is the stainless steel screw, the specimen under analysis.Before inserting the electrode into the corrosion vessel, wipe it with an 80%ethanol soaked wipe, and place it in a glass beaker filled with 100 milliliters of phosphate buffered saline. Use a connection pin to attach the electrode holders onto the electrode suspenders, Fit the electric suspenders into the entry points of the corrosion vessel's lid. Seal the corrosion vessel with a glass reaction lid and clamp to ensure a waterproof seal.The lid of the chamber provides five entry points for the experimental and measurement apparatus. Use a three-electrode configuration, consisting of a reference, counter, and working electrode. For the reference electrode, use a standard silver-silver chloride electrode.For the counter electrode, use a platinum mesh that is loosely bent to wrap around the specimen under test. Once the chamber is sealed with all electrodes placed inside the corrosion vessel, set the temperature to 37 degrees celsius, and open the nitrogen valve with a flow rate of 150 cubic centimeters per minute. Leave the temperature and nitrogen running for 60 minutes before conducting a run.Keep the nitrogen running for the duration of the experiment. Make electrical connections between the potentia stat and the three electrodes, and then turn the potentia stat on. Leave the sample to equilibrate and stabilize within the corrosion vessel environment.The time duration for this varies and is dependent on material. Monitor the potential using measurement view to determine if stabilized conditions are reached. The potentia will be constant with no fluctuations when stable conditions are achieved.Select the cyclic voltammetry potentia stat procedure within the setup view from the procedure tab. Select the option to automate the current range. Set the highest current in the range to be 10 milliamps and the lowest current in the range to be 10 nanoamps for the working electrode.Ensure the final cut off selection is controlled through the potential, by setting the cycle back parameter to 800 millivolts to allow the hysteresis loop to complete. Record the open circuit potential, or OCP, from the measurement view into the OCP parameter text box. Set the start potential 100 millivolts below the recorded OCP value.Set the upper vertex potential to 800 millivolts, the lower vertex to 100 millivolts below the start potential, and the stop potential to 100 millivolts below the lower vertex potential. Then, set the scan rate to 0.001 volts per second, and the step potential to 0.0024 volts per second. Now, press start.To remove the sample from the electrode holder, prepare three small jars of 50 milliliters of dichloromethane under the fume hood. Remove the tested samples from the electrode holders by immersing the lower end of the holder in dichloromethane for 30 minutes inside the fume hood. Once detached, place the specimen into the next jar of dichloromethane and leave for 15 minutes.Repeat this process with the third and final rinse to get rid of any excess coating on the attachment sections of the sample. Shown here, is the representative results of a potentiodynamic polarization scan from a corrosion run for stainless steel 316, which can be further used to extract important corrosion parameters that provide corrosion characterization of the test specimen. This polarization scan shows the direction of the forward and reverse scans.and specifies the point at which the protection potential and pitting potential are observed. The hysteresis loop is reversed at the set potential and returns to intercept the anodic curve, identifying the protection potential. It is critical to have a system that provides high-quality scans, which are reproducible and reliable, since potentiodynamic polarization plots are used to calculate the corrosion current and corrosion potential using tafel extrapolation.Once mastered, this technique can be done in approximately two hours if performed properly. This does not include the time for the sample to corrode, as this time is material dependent. While attempting this procedure, it is important to remember not to contaminate any electrodes and any glassware prior to the corrosion run.Limiting any impurities is critical. Following this procedure, other methods like scanning electron microscopies, surface analysis, or other imaging techniques, or quantification of corrosion techniques, can be performed in order to answer additional questions. After watching this video, you should have a good understanding of how to conduct a reliable electro-dynamic corrosion system.Don't forget that working with microstop laquer can be extremely hazardous, and precautions such as double gloves and working under a film hood, should always be taken while performing this procedure.

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A CO2 Concentration Gradient Facility for Testing CO2 Enrichment and Soil Effects on Grassland Ecosystem Function

A CO2 Concentration Gradient Facility for Testing CO2 Enrichment and Soil Effects on Grassland Ecosystem Function

En CO<sub&gt; 2</sub&gt; Koncentrationsgradient faciliteten for Testing CO<sub&gt; 2</sub&gt; Berigelse og Jord Virkninger på Grassland Ecosystem Funktion

Continuing increases in atmospheric carbon dioxide concentrations (CA) mandate techniques for examining impacts on terrestrial ecosystems. Most ...

Preparation and Testing of Impedance-based Fluidic Biochips with RTgill-W1 Cells for Rapid Evaluation of Drinking Water Samples for Toxicity

Forberedelse og Test af Impedans-baserede strømningstekniske Biochips med RTgill-W1 Celler til hurtig evaluering af drikkevand Prøver til Toksicitet

This manuscript describes how to prepare fluidic biochips with Rainbow trout gill epithelial (RTgill-W1) cells for use in a field portable water toxicity ...

Dispersion of Nanomaterials in Aqueous Media: Towards Protocol Optimization

Spredning af nanomaterialer i vandige medier: mod protokollen optimering

The sonication process is commonly used for de-agglomerating and dispersing nanomaterials in aqueous based media, necessary to improve homogeneity and ...

On the Preparation and Testing of Fuel Cell Catalysts Using the Thin Film Rotating Disk Electrode Method

På forberedelse og afprøvning af Fuel Cell katalysatorer ved hjælp af tynde Film roterende Disk elektrode metode

We present a step-by-step tutorial to prepare proton exchange membrane fuel cell (PEMFC) catalysts, consisting of Pt nanoparticles (NPs) supported on a ...

Protocol for Acute and Chronic Ecotoxicity Testing of the Turquoise Killifish Nothobranchius furzeri

Protocol for Acute and Chronic Ecotoxicity Testing of the Turquoise Killifish Nothobranchius furzeri

Protokol for akut og kronisk Økotoksicitet afprøvning af turkis Killifish Nothobranchius furzeri

The killifish Nothobranchius furzeri is an emerging model organism in the field of ecotoxicology and its applicability in acute and chronic ecotoxicity ...

Bioindication Testing of Stream Environment Suitability for Young Freshwater Pearl Mussels Using In Situ Exposure Methods

Bioindication Testing of Stream Environment Suitability for Young Freshwater Pearl Mussels Using In Situ Exposure Methods

Bioindication afprøvning af Stream miljø egnethed for unge ferskvands perle muslinger med In Situ eksponering metoder

Knowledge of habitat suitability for freshwater mussels is an important step in the conservation of this endangered species group. We describe a protocol ...

Metal Corrosion and the Efficiency of Corrosion Inhibitors in Less Conductive Media

Metal korrosion og effektiviteten af korrosionsinhibitorer i mindre ledende medier

Material corrosion can be a limiting factor for different materials in many applications. Thus, it is necessary to better understand corrosion processes, ...

A Small-Scale Setup for Algal Toxicity Testing of Nanomaterials and Other Difficult Substances

En mindre opsætning for algetoksicitetstest af nanomaterialer og andre vanskelige stoffer

Ecotoxicity data is a requirement for pre- and post-market registration of chemicals by European and international regulations (e.g., REACH). The algal ...