Cardiotoxin Injection in the Tibialis Anterior Muscle
1:29
Tibialis Anterior Isolation
2:51
Fresh Frozen Muscle Technique
4:03
Cryostat Sectioning of Frozen Muscles
4:46
Hematoxylin & Eosin Stains
5:57
Results: Time Course Analysis of Skeletal Muscle Regeneration
7:04
Conclusion
Transcript
The overall goal of this procedure is to obtain a mouse model of skeletal muscle acute injury to study the regeneration process in vivo, from the very early stages to the end of the process. Mouse models of muscle acute injury is a powerful tool t
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Dette håndskrift beskriver en detaljeret protokol til at fremkalde akut skeletmuskulatur regenerering i voksne mus og efterfølgende manipulationer af de muskler, såsom dissektion, frysning, opskæring, rutinemæssige farvning og myofiber tværsnitsareal analyse.