Cardiotoxin Injection in the Tibialis Anterior Muscle
1:29
Tibialis Anterior Isolation
2:51
Fresh Frozen Muscle Technique
4:03
Cryostat Sectioning of Frozen Muscles
4:46
Hematoxylin & Eosin Stains
5:57
Results: Time Course Analysis of Skeletal Muscle Regeneration
7:04
Conclusion
Transcript
The overall goal of this procedure is to obtain a mouse model of skeletal muscle acute injury to study the regeneration process in vivo, from the very early stages to the end of the process. Mouse models of muscle acute injury is a powerful tool t
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Questo manoscritto descrive un protocollo dettagliato per indurre rigenerazione muscolare acuta nei topi adulti e successive manipolazioni dei muscoli, come dissezione, gelati, taglio, colorazione di routine, e myofiber analisi sezione trasversale.