L'utilisation de la Induced somatique analyse sectorielle (AISS) pour l'étude des gènes et de promoteurs impliqués dans la formation du bois et le développement de souches secondaire
Preparation of Agrobacterium tumefaciens for Inoculation
1:59
Inoculation of Plant Stems with A. tumefaciens
3:44
Harvesting for GUS Histological Assay
5:57
Identification of Transgenic Tissue
7:22
Results: Successful Transformation of Stem Tissue Using the ISSA Method
9:12
Conclusion
Transcript
The overall goal of Induced Somatic Sector Analysis is to provide a medium to high throughput technique for the functional characterization of gene and promoter constructs in tree secondary stem tissue within comparatively short time frames. This
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Here we present a protocol that facilitates the medium to high throughput functional characterization of gene and promoter constructs in tree secondary stem tissue within comparatively short time frames. It is efficient, easy to use and widely applicable to a range of tree species.