Genetic Crosses and Collection of Third Instar Larvae
3:10
Dissection of Larvae
4:52
Fixing and Staining of Tissues
5:58
Tissue Mounting and Quantification of Tumor Invasiveness
7:10
Results: Visualization, Tumor Quantification, and Gene Expression
9:04
Conclusion
Transcript
The overall goal of this protocol is to demonstrate how to dissect the drosophila eye-antennal imaginal discs and brains, carrying genetically defined fluorescently-marked clones and how to process them to visualize and quantify gene expression ch
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Denne protokol viser, hvordan til at generere fluorescens mærket, genetisk definerede klonede tumorer i Drosophila øje / den antennal fantasiens diske (EAD). Den beskriver, hvordan at dissekere EAD og hjerne fra den tredje stadiums larver og hvordan at behandle dem til at visualisere og kvantificere genekspression ændringer og tumorindtrængen.