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JoVE Journal

Neuroscience

This content is Open Access.

Subcellular Patch-clamp Recordings from the Somatodendritic Domain of Nigral Dopamine Neurons

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09:17 min

November 2nd, 2016

November 2nd, 2016

14,698 Views

0:05

Title

0:36

Preparation of Brain Slices

2:11

Dendritic Cell-attached Recording

5:58

Post Hoc Visualization of Biocytin-filled Neurons

7:07

Results: Cell-attached Recording in a DA Neuron

8:13

Conclusion

Transcript

Subcellular patch-clamp recordings offer the possibility to investigate the functional properties of dendrites. However these fine structures are not easily accessible due to their small diameter. The protocol described here aims to facilitate the collection of stable and reliable recordings from the dendrites of dopamine neurons in slices in vitro.

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