Cell Surface Staining for Fluorescence-Activated Cell Sorting (FACS)
4:09
Plasmacytoid Cell FACS Purification
5:48
Results: IFNalpha Production by FACS-Sorted pDC
7:10
Conclusion
Transcript
The overall goal of this experiment is to isolate plasmacytoid dendritic cells, or PDC, with a high purity from the bone marrow of lupus-prone mice for the study of their function. This method can help answer key questions related to the character
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Biz pDC fonksiyonel çalışmalar için, lupus-eğilimli farelerin kemik iliğinden, yüksek saflıkta plazmasitoid dendritik hücreleri (PDC) izole etmek için sıralama floresans ile aktive edilmiş hücre kullanarak bir protokol sunulmuştur.