Calibration of Fluorescent Signal Intensity as a Function of Cell Number
2:29
Animal Model of Liver Metastases
5:44
In Vivo Bioluminescent Imaging
7:58
Ex Vivo Fluorescent Imaging
9:05
Results: Metastases and Differential Liver Colonization of Colorectal Cancer Cells
10:34
Conclusion
Transcript
The overall goal of this approach is to model liver colonization by metastatic tumor clones generated by colorectal cancers, and to use this model to improve diagnostics and therapy of liver metastases. Clinical observations indicate the heterogen
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The ability of metastatic clones to colonize distant sites depends on their proliferation capacity and/or their ability to survive in the host microenvironment without significant proliferation. Here, we present an animal model that allows quantitative visualization of both types of liver colonization by metastatic clones.