Isolation of Human Blood-derived Neutrophils by Density Gradient Centrifugation
3:59
Isolate the Lipids from the Human Peripheral Blood-derived Neutrophils
5:33
High Performance Thin Layer Chromatography (HPTLC) to Semi-quantify the Amount of Lipids
7:53
High Performance Liquid Chromatography (HPLC) to Quantify the Amount of Cholesterol
8:48
Results: Isolation of Human Blood-derived Neutrophils and Subsequent Lipid Analysis
9:33
Conclusion
Transcript
The overall goal of this method is to investigate changes in the lipid composition of neutrophils during the release of neutrophil extracellular traps by using thin layer chromatography and HPLC. This method can be used to gain more detailed infor
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Lipids are known to play an important role in cellular functions. Here, we describe a method to determine the lipid composition of neutrophils, with emphasis on the cholesterol level, by using both HPTLC and HPLC to gain a better understanding of the underlying mechanisms of neutrophil extracellular trap formation.