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ツールは、Helix歪曲、部位特異的DNA鎖間架橋の処理に建築タンパク質HMGB1の役割を研究するために

DOI :

10.3791/54678-v

November 10th, 2016

November 10th, 2016

8,114 Views

1Division of Pharmacology and Toxicology, College of Pharmacy, The University of Texas at Austin

Targeted DNA damage can be achieved by tethering a DNA damaging agent to a triplex-forming oligonucleotide (TFO). Using modified TFOs, DNA damage-specific protein association, and DNA topology modification can be studied in human cells by the utilization of modified chromatin immunoprecipitation assays and DNA supercoiling assays described herein.

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117 DNA DNA ChIP 2D HMGB1

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