T Cell Receptor (TCR) Hemichain-encoded Retroviral Construct Preparation
1:41
Stable Packaging Cell Line Generation
3:08
Human T Cell Activation and Transduction
5:04
Novel Antigen-specific TCR Clone Reconstitution
6:25
Results: Centric TCR Hemichains Pair with Different Counter-chains to Form Antigen-specific TCRs with Varying Affinity
7:59
Conclusion
Transcript
The overall goal of this experimental procedure is to generate De Novo T Cell Receptors or TCRS, recognizing a specific antigen of interest. This method can help isolate optimal T Cell Receptors in a field of gene engineered immunotherapy. The mai
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Heri vi beskrive en ny fremgangsmåte for å generere antigen-spesifikke T-celle-reseptorer (TCR) etter sammenkobling TCRα eller TCRβ av en eksisterende TCR, som har antigen-spesifisitet av interesse, med komplementære hemichain av den perifere T-cellereseptoren repertoar. De novo generert TCR beholde antigen-spesifisitet med varierende affinitet.