T Cell Receptor (TCR) Hemichain-encoded Retroviral Construct Preparation
1:41
Stable Packaging Cell Line Generation
3:08
Human T Cell Activation and Transduction
5:04
Novel Antigen-specific TCR Clone Reconstitution
6:25
Results: Centric TCR Hemichains Pair with Different Counter-chains to Form Antigen-specific TCRs with Varying Affinity
7:59
Conclusion
Transcript
The overall goal of this experimental procedure is to generate De Novo T Cell Receptors or TCRS, recognizing a specific antigen of interest. This method can help isolate optimal T Cell Receptors in a field of gene engineered immunotherapy. The mai
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Häri beskriver vi en ny metod för att generera antigenspecifika T-cellreceptorer (TCR) genom att para ihop den TCRα eller TCRβ av en befintlig TCR, som har den antigenspecificitet av intresse, med komplementär hemichain av den perifera T-cell-receptorrepertoar. De novo genererade TCRs behålla antigen-specificitet med varierande affinitet.