Characterization of Drug Resistance Profiles through Cytotoxicity Assays
5:01
RNA Isolation and Library Preparation for RNA-sequencing
5:55
Validation of the Results by RT-PCR and qRT-PCR Assays
7:21
Results: Detection of Differential Splicing Events in Chemotherapy-resistant Cell Lines Compared to Parental Wild-type by Using RNA-sequencing
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Conclusion
Transcript
The overall goal of this protocol is to investigate the association of aberrant splicing with drug resistance profiles in in vitro models of solid tumors and hematological malignancies. This method can help answer key question in the field of mole
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Her beskriver vi en protokoll for å undersøke virkningen av avvikende spleising på legemiddelresistens i solide svulster og hematologisk malignitet. Til dette målet, analyserte vi transcriptomic profiler av foreldre og resistente in vitro modeller gjennom RNA-seq og etablert en QRT-PCR basert metode for å validere kandidat gener.