Characterization of Drug Resistance Profiles through Cytotoxicity Assays
5:01
RNA Isolation and Library Preparation for RNA-sequencing
5:55
Validation of the Results by RT-PCR and qRT-PCR Assays
7:21
Results: Detection of Differential Splicing Events in Chemotherapy-resistant Cell Lines Compared to Parental Wild-type by Using RNA-sequencing
9:07
Conclusion
Transcript
The overall goal of this protocol is to investigate the association of aberrant splicing with drug resistance profiles in in vitro models of solid tumors and hematological malignancies. This method can help answer key question in the field of mole
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Aqui nós descrevemos um protocolo teve como objetivo investigar o impacto do splicing aberrante na resistência aos medicamentos em tumores sólidos e doenças malignas hematológicas. Para este objetivo, foram analisados os perfis transcriptomic de modelos parentais e resistentes in vitro através de RNA-seq e estabeleceu um método baseado qRT-PCR para validar genes candidatos.