Characterization of Drug Resistance Profiles through Cytotoxicity Assays
5:01
RNA Isolation and Library Preparation for RNA-sequencing
5:55
Validation of the Results by RT-PCR and qRT-PCR Assays
7:21
Results: Detection of Differential Splicing Events in Chemotherapy-resistant Cell Lines Compared to Parental Wild-type by Using RNA-sequencing
9:07
Conclusion
Transcript
The overall goal of this protocol is to investigate the association of aberrant splicing with drug resistance profiles in in vitro models of solid tumors and hematological malignancies. This method can help answer key question in the field of mole
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Aquí se describe un protocolo destinado a investigar el impacto de corte y empalme aberrante de resistencia a fármacos en tumores sólidos y neoplasias hematológicas. Para este objetivo, se analizaron los perfiles de transcriptómica de modelos parentales y resistentes in vitro a través de RNA-seq y establecimos un método basado QRT-PCR para validar los genes candidatos.