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An Alternative Approach for Sample Preparation with Low Cell Number for TEM Analysis

13.4K Views

09:17 min

October 12th, 2016

October 12th, 2016

13,403 Views

0:05

Title

0:41

Cell Fixation, Staining, and Pre-embedding

3:54

Osmification, Dehydration, and Embedding

5:22

Sectioning and Transmission Electron Microscopy

7:32

Results: Transmission Electron Analysis of Low Cell Number Samples

8:37

Conclusion

Transcript

The overall goal of this procedure is to prepare samples with low cell numbers to perform TEM analysis in an efficient and reproducible manner. This method can help to reveal all structures of real cells and a better physiological settings in the

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Here, we present a protocol to prepare samples with low cell numbers for transmission electron microscopy (TEM) analysis.

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