Preparation and Cultivation of Primary Cortical Astrocytes
5:19
Preparing Astrocytes for the Indirect Coculture
6:48
Preparation of Primary Hippocampal Neurons
9:03
Results: The Indirect, Astrocyte-neuron Coculture System Allows for Separate Analysis of Astrocytes, Neurons and Secreted Molecular Mediators
10:01
Conclusion
Transcript
The overall goal of this Indirect Coculture Assay, is to investigate the impact of astrocytes on the development of neurons. Our laboratory is interested in the role of neuron-glia interactions. It is believed that astrocytes contribute to the for
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This protocol describes the indirect neuron-astrocyte coculture for compartmentalized analysis of neuron-glia interactions.