Preparation of PEGylated Antibody (PEG-Ab) Solution
2:25
Prepare UV-Vis/Raman Probes
4:57
Immunoassay Plate Preparation
6:08
Functionalizing the Immunoassay Plate
7:18
Test Assay Sensitivity by UV-Vis and Raman Spectroscopy
7:58
Results: Determining the Sensitivity of UV-Vis and Raman Spectroscopy
8:39
Conclusion
Transcript
The overall goal of this protocol is to demonstrate the development of a customizable gold nano particle based immunoassay capable of analysis using UV-Visible and Raman Spectroscopy. The use of two optical methods provides internal assay validati
Sign in or start your free trial to access this content
Nanoparticle-based optical probes have been designed as a vehicle for detecting antigens using Raman and UV-Vis spectroscopy. Here we describe a protocol for preparing such probes for a UV-Vis/Raman spectroscopy immunoassay in such a way to incorporate future multiplexing capabilities.