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DOI :
10.3791/54799-v
March 3rd, 2017
Chapters
0:05
Title
0:41
Cell Culture and HIV-1 Infection
2:11
Exosome Isolation
3:42
Protein Extraction
4:58
Proteomic Data Analysis
5:43
Bioinformatics Verification and Characterization
8:20
Results: SILAC Is an Effective Method for Analyzing the Effects of HIV-1 Infection on Host Exosomal Proteomes
9:08
Conclusion
ここでは、ホストエキソソームプロテオームのHIV-1感染の効果を分析するために、細胞培養中のアミノ酸による安定同位体標識法(SILAC)を用いて定量的プロテオミクスの方法を記載します。このプロトコルは、容易に異なるストレスまたは感染の条件下で細胞に適合させることができます。
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