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JoVE Journal

Immunology and Infection

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从全血人单核细胞来源的树突状细胞的生成

19.9K Views

07:35 min

December 24th, 2016

December 24th, 2016

19,928 Views

0:05

Title

0:51

Peripheral Blood Mononuclear Cell (PBMC) Enrichment

2:30

Monocytes Anti-human CD14 Magnetic Particle Isolation

4:08

Monocyte Interleukin (IL)-4 and Granulocyte Macrophage–Colony Stimulating Factor (GM-CSF) Stimulation

5:10

Results: Representative Human Monocyte–Dendritic Cell Generation from Whole Blood

6:19

Conclusion

Transcript

The overall goal of this CD14 magnetic beads separation technique is to isolate monocytes from the blood for their further differentiation into dendritic cells or macrophages in vitro. This method can help answer key questions in the field of immu

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Here, we demonstrate how monocytes are isolated by magnetic bead separation from peripheral blood mononuclear cells after density gradient centrifugation of human anti-coagulated blood. Following incubation for 5 days, human monocytes are differentiated into immature dendritic cells and are ready for experimental procedures in a non-clinical setting.

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