JoVE Logo
Faculty Resource Center

Sign In

Genereren van Human-monocyten afgeleide dendritische cellen uit volbloed

DOI :

10.3791/54968-v

December 24th, 2016

December 24th, 2016

19,573 Views

1Division of Hygiene and Medical Microbiology, Medical University of Innsbruck

Here, we demonstrate how monocytes are isolated by magnetic bead separation from peripheral blood mononuclear cells after density gradient centrifugation of human anti-coagulated blood. Following incubation for 5 days, human monocytes are differentiated into immature dendritic cells and are ready for experimental procedures in a non-clinical setting.

Tags

Immunologie

-- Views

Related Videos

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved