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DOI :
10.3791/55000-v
April 18th, 2017
Chapters
0:00
Title
17:01
Conclusion
1:19
Glia Culture Preparation
5:57
Preparing Coverslips for Neuron Culture
9:22
Neuronal Culture Preparation
16:13
Immunofluorescence Results for Neuronal Markers
本文介绍用于培养低密度初级海马神经元上倒置在神经胶质单层玻璃盖玻片生长的协议。神经元和神经胶质层通过石蜡珠分离。通过该方法生长的神经元适合于高分辨率光学成像和功能测定法。
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