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JoVE Journal

Neuroscience

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低密度初代海馬ニューロンの文化

20.2K Views

17:46 min

April 18th, 2017

April 18th, 2017

20,213 Views

0:00

Title

17:01

Conclusion

1:19

Glia Culture Preparation

5:57

Preparing Coverslips for Neuron Culture

9:22

Neuronal Culture Preparation

16:13

Immunofluorescence Results for Neuronal Markers

Transcript

This protocol was originally published by Dr.Gary Bunker and his colleagues, and they allow for the long density and the high purity culture of embryonic loading hippocampal neurons, by suspending the neurons over a glial fitter layer. This result

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この記事では、グリア単層の上に反転したカバーガラス上で成長する低密度初代海馬神経細胞を培養するためのプロトコルを記述しています。ニューロンとグリアの層は、パラフィンワックスビーズで分離されています。この方法によって成長させた神経細胞は、高分解能光学イメージングおよび機能アッセイに適しています。

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