JoVE Logo
Faculty Resource Center

Sign In

In Vitro Differentiation of Human CD4+FOXP3+ Induced Regulatory T Cells (iTregs) from Naïve CD4+ T Cells Using a TGF-β-containing Protocol

DOI :

10.3791/55015-v

December 30th, 2016

December 30th, 2016

19,272 Views

1Unit of Computational Medicine, Center for Molecular Medicine, Department of Medicine Solna, Karolinska Institutet, Karolinska University Hospital, & Science for Life Laboratory

This protocol describes the reproducible generation and phenotyping of human induced regulatory T cells (iTregs) from naïve CD4+ T cells in vitro. Different protocols for FOXP3 induction allow for the study of specific iTreg phenotypes obtained with respective protocols.

-- Views

Related Videos

article

Human T Lymphocyte Isolation, Culture and Analysis of Migration In Vitro

article

Following Cell-fate in E. coli After Infection by Phage Lambda

article

Determining Optimal Cytotoxic Activity of Human Her2neu Specific CD8 T cells by Comparing the Cr51 Release Assay to the xCELLigence System

article

Induction of Graft-versus-host Disease and In Vivo T Cell Monitoring Using an MHC-matched Murine Model

article

piggyBac Transposon System Modification of Primary Human T Cells

article

Peptide:MHC Tetramer-based Enrichment of Epitope-specific T cells

article

Clinical Application of Sleeping Beauty and Artificial Antigen Presenting Cells to Genetically Modify T Cells from Peripheral and Umbilical Cord Blood

article

Real-time Live Imaging of T-cell Signaling Complex Formation

article

New Tools to Expand Regulatory T Cells from HIV-1-infected Individuals

article

Adenoviral Transduction of Naive CD4 T Cells to Study Treg Differentiation

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved