Research
Education
Sign In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Please note that all translations are automatically generated. Click here for the English version.
DOI :
10.3791/55035-v
January 8th, 2017
Chapters
0:05
Title
0:49
Mouse Embryonic Stem Cell (mESC) Culture
2:06
Serum- and Feeder-free Conditioned Medium Collection
3:17
Senescence-associated (SA) Beta Galactosidase Assay
4:46
Cell Cycle and qRT-PCR Analyses
7:19
Results: Characterization of the Effects of mESC-conditioned Medium on Cell Growth, Cycle Stage, and Function
8:29
Conclusion
这个协议提供了小鼠胚胎干细胞的血清衍生的集合(MESC)-conditioned培养基(MESC-CM)的方法(胎牛血清,FBS) - 和馈线(小鼠胚胎成纤维细胞,MEF中)用于小区-free条件 - 免费的做法。它可以是适用于老化和老化有关的疾病的治疗。
Tags
-- Views
Related Videos
Privacy
Terms of Use
Policies
Contact Us
Recommend to library
JoVE NEWSLETTERS
JoVE Journal
Methods Collections
JoVE Encyclopedia of Experiments
Archive
JoVE Core
JoVE Business
JoVE Science Education
JoVE Lab Manual
Faculty Resource Center
Authors
Overview
Publishing Process
Editorial Board
Scope and Policies
Peer Review
FAQ
Submit
Librarians
Testimonials
Subscriptions
Access
Resources
Library Advisory Board
ABOUT JoVE
Leadership
Copyright © 2024 MyJoVE Corporation. All rights reserved