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Preparation and Culture of Myogenic Precursor Cells/Primary Myoblasts from Skeletal Muscle of Adult and Aged Humans

DOI :

10.3791/55047-v

10:10 min

February 16th, 2017

February 16th, 2017

21,185 Views

1Institute of Ageing and Chronic Disease, University of Liverpool, 2Aintree University Hospital

This protocol describes a robust, reproducible and simple method of isolation and culture of myoblast progenitor cells from the skeletal muscle of adult and aged people. The muscles used here include foot and leg muscles. This approach enables the isolation of an enriched population of primary myoblasts for functional studies.

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Preparation

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