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JoVE Journal

Medicine

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Analyzing Beneficial Effects of Nutritional Supplements on Intestinal Epithelial Barrier Functions During Experimental Colitis

12.1K Views

08:58 min

January 5th, 2017

January 5th, 2017

12,092 Views

0:05

Title

0:57

Nutritional Supplement Application by Gavage and Disease Activity Index (DAI) Determination

2:14

Evans Blue Assay

3:41

Tissue Collection, Colon Histology Staining Preparation, and Oxidative Stress Analysis

6:10

Results: Representative Nutritional Supplements Effects on Intestinal Epithelial Barrier Function During Experimental Colitis

7:59

Conclusion

Current treatments of inflammatory bowel diseases (IBD) aim at alleviating disease symptoms but may cause severe side effects. Thus, alternative strategies are being investigated in animal colitis models. Here, we explain how the beneficial effects of diet supplements on clinical IBD signs are analyzed in such a colitis model.

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