تحليل البروتينات ملزم كاب في خلايا الإنسان تتعرض لظروف الأوكسجين فسيولوجية

Here, we present human cell culture protocols to analyze translation initiation factors that bind the 5' cap of mRNA during physiological oxygen conditions. This method utilizes an Agarose-linked m⁷GTP cap analog and is suitable to investigate cap-binding factors and their interacting partners.