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JoVE Journal

Developmental Biology

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Production and Administration of Therapeutic Mesenchymal Stem/Stromal Cell (MSC) Spheroids Primed in 3-D Cultures Under Xeno-free Conditions

11.7K Views

11:05 min

March 18th, 2017

March 18th, 2017

11,692 Views

0:05

Title

1:25

MSC Expansion and Preparation of Activated MSC Spheroids in 3-D Hanging Drop Cultures

4:50

Intraperitoneal Delivery of Intact Spheroids

8:02

Results: Spheroids Produced Using MSC Hanging Drop Cultures

9:31

Conclusion

Transcript

The overall goal of these experiments is to efficiently generate and inject therapeutic spherical aggregates, or spheroids, of mesenchymal stem/stromal cells, or MSCs, primed under xeno-free conditions in experimental and clinical applications. Th

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The therapeutic potential of mesenchymal stem/stromal cells (MSCs) is well-documented, however the best method of preparing the cells for patients remains controversial. Herein, we communicate protocols to efficiently generate and administer therapeutic spherical aggregates or 'spheroids' of MSCs primed under xeno-free conditions for experimental and clinical applications.

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