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DOI :
10.3791/55134-v
January 13th, 2017
Chapters
0:05
Title
0:51
Mouse Embryonic Gonadal Ridge Isolation
3:06
Embryonic Gonadal Ridge Culture
4:38
Whole Mount Gonadal Ridge Immunofluorescence
6:42
Results: Wolffian Duct Morphogenesis and Surface Marker Expression
7:40
Conclusion
我々は、マウスウォルフ管(WD)の単離および培養のための方法を提示します。また、蛍光タグ付けされた抗体を用いて培養/単離されたばかりのWDSのホールマウント免疫染色のための詳細な手順を示しています。一緒に、これらの技術は、WDの開発、コイリング、および分化の研究を可能にします。
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