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Developmental Biology

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Organ Culture and Whole Mount Immunofluorescence Staining of Mouse Wolffian Ducts

10.6K Views

09:16 min

January 13th, 2017

January 13th, 2017

10,617 Views

0:05

Title

0:51

Mouse Embryonic Gonadal Ridge Isolation

3:06

Embryonic Gonadal Ridge Culture

4:38

Whole Mount Gonadal Ridge Immunofluorescence

6:42

Results: Wolffian Duct Morphogenesis and Surface Marker Expression

7:40

Conclusion

Transcript

The overall goal of this organ culture and whole mount immunofluorescene method is to better visualize and understand the process of Wolffian induct coiling and development. This method will help to answer key questions in the field of development

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We present a method for isolation and culture of the mouse Wolffian duct (WD). We also demonstrate a detailed procedure for whole mount immunostaining of cultured/freshly isolated WDs with fluorescently tagged antibodies. Together, these techniques enable the study of WD development, coiling, and differentiation.

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