Genom-dækkende analyse af HDAC-hæmmer-medieret graduering af MicroRNA og mRNAs i B celler inducerede undergår klasse-skifte DNA rekombination og Plasma Celledifferentiering
Stimulation of Mouse B Cells for Class-switch DNA Recombination (CSR), Plasma Differentiation and HDI Treatment
3:04
B-cell Isolation
4:26
B-cell Stimulation and HDI Treatment
5:17
Flow Cytometry Analysis of CSR and Plasma Cell Differentiation
7:11
Quantitative RT-PCR of mRNA and miRNAs
8:41
Results: Analysis of Epigenetic Modulation of miRNA and Gene Expression in B Cells
10:28
Conclusion
Transcript
The overall goal of this experiment is to induce B lymphocytes to undergo antibody class-switching and plasma cell differentiation and analyze the modulation as well as epigenetic regulation by the expression of HDAC inhibitor mRNA and microRNA in
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Epigenetiske faktorer kan interagere med genetiske programmer til at modulere genekspression og regulere B-cellefunktion. Ved at kombinere in vitro B-celle stimulation, qRT-PCR, og høj overførselshastighed mikroRNA-sekvens og mRNA-sekvensen tilgange, kan vi analysere den epigenetiske graduering af miRNA og gen udtryk i B-celler.