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DOI :
10.3791/55135-v
September 20th, 2017
Chapters
0:05
Title
0:56
Stimulation of Mouse B Cells for Class-switch DNA Recombination (CSR), Plasma Differentiation and HDI Treatment
3:04
B-cell Isolation
4:26
B-cell Stimulation and HDI Treatment
5:17
Flow Cytometry Analysis of CSR and Plasma Cell Differentiation
7:11
Quantitative RT-PCR of mRNA and miRNAs
8:41
Results: Analysis of Epigenetic Modulation of miRNA and Gene Expression in B Cells
10:28
Conclusion
後成の要因は遺伝子発現を調節して B 細胞の機能を調節する遺伝的プログラムと対話できます。体外B 細胞刺激、qRT PCR とハイスループット マイクロ Rna シーケンスおよび mRNA シーケンスのアプローチを組み合わせて、B 細胞のマイクロ Rna と遺伝子発現のエピジェネティックな変調を分析できます。
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