January 24th, 2017
•This protocol describes the production of a therapeutic antibody in a mammalian expression system. The methods described include preparation of vector DNA, stable transfection and serum-free adaptation of a human embryonic kidney 293 cell line, set up of large scale cultures and purification using affinity chromatography.
Related Videos
Directed Protein Packaging within Outer Membrane Vesicles from Escherichia coli: Design, Production and Purification
Production, Crystallization and Structure Determination of C. difficile PPEP-1 via Microseeding and Zinc-SAD
Expression, Purification, and Antimicrobial Activity of S100A12
A Guide to Production, Crystallization, and Structure Determination of Human IKK1/α
Method for Efficient Refolding and Purification of Chemoreceptor Ligand Binding Domain
Purification and Reconstitution of TRPV1 for Spectroscopic Analysis
Transient Expression in Nicotiana Benthamiana Leaves for Triterpene Production at a Preparative Scale
Large-scale Production of Recombinant RNAs on a Circular Scaffold Using a Viroid-derived System in Escherichia coli
Expression and Purification of Nuclease-Free Oxygen Scavenger Protocatechuate 3,4-Dioxygenase
A Customizable Approach for the Enzymatic Production and Purification of Diterpenoid Natural Products
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved