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JoVE Journal

Genetics

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Detection of Inter-chromosomal Stable Aberrations by Multiple Fluorescence In Situ Hybridization (mFISH) and Spectral Karyotyping (SKY) in Irradiated Mice

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10:14 min

January 11th, 2017

January 11th, 2017

9,751 Views

0:05

Title

0:53

Isolation of Bone Marrow Mononuclear Cells by Density Gradient Centrifugation and Preparation of Metaphase Cell Spreads

3:04

Mouse Chromosome Painting by mFISH

6:07

Spectral Karyotyping of Mouse Chromosomes

7:45

Results: Inter-chromosomal Stable Aberration Analysis by Multiple Fluorescence In Situ Hybridization and Spectral Karyotyping

9:02

Conclusion

Transcript

The overall goal of this molecular cytogenic method is to observe interchromosomal stable aberrations in the bone marrow cells of mice exposed to total body irradiation. This method can help answer the key question in the radiation biology field s

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The present protocol describes the usefulness of multiple fluorescence in situ hybridization (mFISH) and spectral karyotyping (SKY) in identifying inter-chromosomal stable aberrations in the bone marrow cells of mice after exposure to total body irradiation.

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